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典型應(yīng)用

     活體體內(nèi)光學(xué)成像(Optical in vivo Imaging)具有操作簡(jiǎn)單、所得結(jié)果直觀、靈敏度高等特點(diǎn),因?yàn)橐呀?jīng)廣泛應(yīng)用于生命科學(xué),醫(yī)學(xué)研究以及藥物開發(fā)等發(fā)面。該技術(shù)應(yīng)用于植物研究方面,具體的應(yīng)用領(lǐng)域包括:植物的突變體篩選、植物抗逆性狀研究、離體細(xì)胞的基因表達(dá)、植物生理節(jié)律研究、植物發(fā)育研究、蛋白互作等。  

      Lumazone植物活體分子成像系統(tǒng)在植物研究方面典型應(yīng)用案例如下:

>> 突變體篩選                                                                >> 逆境處理研究       

     


>> 蛋白互作研究                                                           >> 基因誘導(dǎo)表達(dá)研究


        


 >> 節(jié)律相關(guān)研究                                                            >> 甲基化研究     


        


發(fā)表文獻(xiàn)

RNA helicase-like protein as an early  regulator  of  transcription factors for plant chilling and freezing tolerance

PNAS 9911507–11512August 20, 2002

Arabidopsis hot Mutants Define Multiple Functions Required for Acclimation to High Temperatures

Plant Physiology 132757–767  Jun 2003

Screening for Gene Regulation Mutants by Bioluminescence 

Imaging

Sci. STKE 140, pl10. (9 July 2002)

Flagellin induces innate immunity in nonhost interactions that is suppressed by Pseudomonas syringae effectors

PNAS 10212990–12995Sep 2005

Arabidopsis Protein Kinase PKS5 Inhibits the Plasma Membrane 

H1-ATPase by Preventing Interaction with 14-3-3 Protein

PLANT CELL 19: 1617 - 1634.( May 2007

Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants

Plant Physiology 146: 368 - 376.( Feb 2008

C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic 

stress signaling, growth, and development

PNAS 99 10893–10898 Aug 2002 

RAR1, a central player in plant immunity, is targeted by Pseudomonas syringae effector AvrB

PNAS 10319200–19205Dec 2006

STABILIZED1, a Stress-Upregulated Nuclear Protein, Is Required for Pre-mRNA Splicing, mRNA Turnover, and 

Stress Tolerance in Arabidopsis

The Plant Cell 18 1736–1749  Jul 2006

FIONA1 Is Essential for Regulating Period Length in the Arabidopsis Circadian Clock

The Plant Cell 20: 307–319,(Feb 2008)

The Plant Cell 20: 307–319,Feb 2008

Interaction of Osmotic Stress, Temperature, and Abscisic Acid in the Regulation of Gene Expression in Arabidopsis

Plant Physiology    119 205–211 Jan 1999

Regulated Expression of Arabidopsis Phosphate Transporters1

Plant Physiology 130 221–233  Sep 2002

Regulation   of   Osmotic  Stress-responsive  Gene  Expression  

by   the LOS6/ABA1 Locus in Arabidopsis

THE JOURNAL OF BIOLOGICAL CHEMISTRY 2778588–8596 

Mar 2002

A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development

Genes Dev. 23: 2717-2722Oct 2009

FIERY1 encoding an inositol polyphosphate 1-phosphatase  is anegative regulator of abscisic acid and stress signaling in 

Arabidopsis

Genes & Dev 15: 1971-1984Jul 2001

A DEAD Box RNA Helicase Is Essential for mRNA Export and Important for Development and Stress Responsesin Arabidopsis

The Plant Cell 17256–267Jan    2005

Genetic Analysis of Osmotic and Cold Stress Signal Transduction 

in Arabidopsis: lnteractions and Convergence of Abscisic Acid-Dependent and Abscisic Acid-lndependent Pathways

The Plant Cell 91935-1949,  Nov 1997

A Peroxidase Contributes to ROS Production during Arabidopsis Root Response to Potassium

Deficiency

Molecular Plant    3420–427  Mar 2010

Activation of a COl1-dependent pathway in Arabidopsis 

by Pseudomonas syringae typeIII effectors and coronatine

The Plant Journal    37589-602  Jan 2004

A Putative Arabidopsis Nucleoporin, AtNUP160, Is Critical for RNA Export and Required for Plant Tolerance to Cold Stress

MOLECULAR AND CELLULAR BIOLOGY 269533–9543 Dec.2006

Regulated Expression of Arabidopsis Phosphate Transporters

Plant Physiology 130: 221 - 233  Sep 2002

COP1-Mediated Ubiquitination of CONSTANS Is Implicated in Cryptochrome Regulation of Flowering in Arabidopsis

The Plant Cell, Vol. 20: 292–306, February 2008

Ethanol breaks dormancy of the potato tuber apical bud

Journal of Experimental Botany 562515–2525Sep 2005

Disruption of Arabidopsis CHY1 Reveals an Important Role of Metabolic Status in Plant Cold

Molecular Plant    259–72  January 2009

HOS10 encodes an R2R3-type MYB transcription factor essential for cold acclimation in plants

PNAS 1029966–9971  Jul 12, 2005

Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance

PNAS 1054945–4950  Mar 2008

NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V 

and is required for RNA-directed DNA methylation

GENES & DEVELOPMENT 23:318–330Jan 2009

Role of the Arabidopsis DNA glycosylase_lyase ROS1 in active DNA demethylation

PNAS 10311796–11801Aug 2006  

A GUS/Luciferase Fusion Reporter for Plant Gene Trapping and for Assay of Promoter Activity with Luciferin-Dependent Control of the Reporter Protein Stability

Plant Cell Physiol. 48(8): 1121–1131 (2007)

Isolation and Characterization of cul1-7, a Recessive Allele of CULLIN1 That Disrupts SCF Function at the C Terminus of CUL1 in Arabidopsis thaliana

Genetics 181: 945–963 (March 2009)

The Plant Cuticle Is Required for Osmotic Stress Regulation of Abscisic Acid Biosynthesis and Osmotic Stress Tolerance in Arabidopsis

PLANT CELL, May 2011; 23: 1971 - 1984

Testing Time: Can Ethanol-Induced Pulses of Proposed Oscillator Components Phase Shift Rhythms in Arabidopsis?

JOURNAL OF BIOLOGICAL RHYTHMS, Vol. 23 No. 6, December 2008 463-471

Fully Codon-Optimized luciferase Uncovers Novel Temperature Characteristics of the Neurospora Clock

EUKARYOTIC CELL, Jan. 2008, p. 28–37

RTE1 Is a Golgi-Associated and ETR1-DependentNegative Regulator of Ethylene Responses

Plant Physiology, September 2007, Vol. 145, pp. 75–86




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